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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production

Fig. 2

Transcriptional upregulation of PrpsJ in response to cell growth in various sub-lethal doses of chloramphenicol, measured as a correlation of PrpsJ-dependent expression of YFP activity and growth rate (a) or rplC transcript accumulation (b). The growth rates were calculated based on change in OD600nm values measured every 1 h over a period of 3 h after adding chloramphenicol. OD600nm normalized fluorescence values were calculated from measurements taken 3 h after adding chloramphenicol. RNA samples collection was performed after 15-min incubation of the recombinant cells in the presence of chloramphenicol. Relative gene expression (ΔΔCt) is presented as the level of rplC transcript in samples treated with chloramphenicol (2, 4, 8 μM) relative to its abundance in reference samples (0 μM). The data presented are from three independent biological replica (average ± SD)

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Microbial Cell Factories

ISSN: 1475-2859

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