Fig. 2From: Developing a single strain for in vitro salvage synthesis of NAD+ at high temperatures and its potential for bioconversionDetermination of gene order in an artificial operon. a The scheme to calculate ideal mRNA amounts for each gene is illustrated. “Ideal mRNA ratio (E)” can be calculated from “Enzyme activity per mRNA (D)” and “Optimal enzyme activity ratio (A)”. b Experimentally determined values are shown. Values are normalized to that of the gene encoding NAMaseBack to article page