Table 1 Strains and plasmids used in this study

Strains

 L. acidophilus ATCC4356

Source of constitutive promoter P_slpA

DSMZ (Brauschweig, Germany)

 L. plantarum WCFS1

Host strain

[30]

 E. coli NEB5α

Host strain

New England Biolabs

Plasmids

 pSIP409GusA

Erm^r; spp- based expression vector pSIP409

[40]

 pEV

Erm^r; pLp_2578sAmyA derivative, no signal sequence, no man (negative control)

[27]

 pUC57

Amp^r; plasmid cloning vector in E. coli containing the synthetic constitutive promoter pgm

GenScript

 pSIP_1261ManB

Erm^r; pLp_1261InvS derivative with fragment of man-myc instead of the inv gene

[22]

 pPgm_1261ManB

Erm^r; pSIP_1261ManB derivative with fragment of constitutive promoter P_pgm instead of inducible promoter P_sppQ in pSIP409 vector

This study

 pSlpA_1261ManB

Erm^r; pSIP_1261ManB derivative with fragment of constitutive promoter P_slpA instead of inducible P_sppQ in pSIP409 vector

This study