Table 1 Strains used in this study
Strains | Properties | Reference |
|---|---|---|
Strains | ||
E. coli JM109 | endA1, recA1, thi, gyrA96, supE44, hsdR17 Δ (lac-proAB)/F′ [traD36, lacIq, lacZΔM15, proAB+] | Takara |
E. coli BL21 | F−, ompT, hsd SB (rB−mB−), gal, dcm | Takara |
B. subtilis RIK1285 | Marburg 168 derivative: trpC2, lys1, aprE Δ3, nprR2, nprE18 | Takara |
B. subtilis RIK1285AS | B. subtilis RIK1285 containing plasmid pBE-SPAprE-amyS | This study |
B. subtilis WS11 | WS4 derivative, ΔwprA, ΔnprB, Δvpr, Δepr, Δbpr, Δmpr, ΔaprE | [23] |
B. subtilis WHS11 | WS11 derivative, ΔhrcA | Our laboratory |
WS11QS | B. subtilis WS11 containing plasmid pHYQamyS | This study |
WS11QSP | B. subtilis WS11 containing plasmid pHYQamySP | This study |
WS11RS | B. subtilis WS11 containing plasmid pHYRamyS | This study |
WS11AS | B. subtilis WS11 containing plasmid pHYAamyS | This study |
WS11YS | B. subtilis WS11 containing plasmid pHYYamyS | This study |
WS11YSP | B. subtilis WS11 containing plasmid pHYYamySP | This study |
WHS11YS | B. subtilis WHS11 containing plasmid pHYYamyS | This study |
WHS11YSA | B. subtilis WHS11 containing plasmid pHYYamySA | This study |