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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection

Fig. 3

Expression and secretion of α-amylase by B. subtilis WS11 variants. a Alpha-amylase activity in the shake-flask culture supernatants obtained using B. subtilis WS11 variants. b SDS-PAGE analysis of the culture supernatants (lanes 1, 4, and 7), cell disruption supernatants (lanes 2, 5, and 8), and cell disruption precipitates (lanes 3, 6, and 9) obtained using B. subtilis strains WS11RS, WS11AS, and WS11YS, respectively. Lane M contains the medium molecular weight protein standard. Error bars indicate the standard deviation from the mean of the three experimental data replicates

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Microbial Cell Factories

ISSN: 1475-2859

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