Table 1 Bacterial strains and plasmids used in this study
Strain or plasmid | Description | Sources |
|---|---|---|
Strains | ||
E. coli DH5α | Lab stock | |
C. glutamicum ATCC13032 | Wild type | Lab stock |
C. glutamicum CGMCCl.15647 | Wild type | Lab stock |
ΔcspB2 | C. glutamicum CGMCCl.15647, cspB2 disruption | This work |
ΔclpS | C. glutamicum CGMCCl.15647, clpS disruption | Lab stock |
ΔcspB2ΔclpS | C. glutamicum CGMCCl.15647, cspB2 and clpS disruption | This work |
ΔcspB2InX | C. glutamicum CGMCCl.15647, cspB2 disruption, xynA integration | This work |
ΔcspB2ΔclpSInX | C. glutamicum CGMCCl.15647, cspB2 disruption, clpS disruption, xynA integration | This work |
Cg47+P19-0 | C. glutamicum CGMCCl.15647 harboring pXMJ19 | This work |
Cg47+P19-X | C. glutamicum CGMCCl.15647 harboring pXMJ19-xynA | This work |
Cg47+P19-cspB1X | C. glutamicum CGMCCl.15647 harboring pXMJ19-cspB1-cspA-xynA | This work |
Cg32+P19-X | C. glutamicum ATCC13032 harboring pXMJ19-xynA | This work |
ΔcspB2+P19-X | ΔcspB2 harboring pXMJ19-xynA | This work |
ΔclpS+P19-X | ΔclpS harboring pXMJ19-xynA | This work |
ΔcspB2ΔclpS+P19-X | ΔcspB2ΔclpS harboring pXMJ19-xynA | This work |
ΔcspB2InX+P19-X | ΔcspB2InX harboring pXMJ19-xynA | This work |
ΔcspB2ΔclpSInX+P19-X | ΔcspB2ΔclpSInX harboring pXMJ19-xynA | This work |
ΔcspB2ΔclpSInX+P19-X+pEC-X | ΔcspB2ΔclpSInX harboring pXMJ19-xynA and pEC-XK99E-xynA | This work |
Plasmids | ||
pXMJ19 (P19-0) | E. coli–C. glutamicum shuttle vector, Chlr | Lab stock |
pXMJ19-cspB2 (P19-cspB2) | pXMJ19 derivative, PcspB2, cspB2 signal peptide, cspB2 gene | |
pXMJ19-cspB1-cspA-xynA (P19-cspB1X) | PcspB1, cspA signal peptide, xynA | This work |
pXMJ19-xynA (P19-X) | pXMJ19 derivative, PcspB2, cspB2 signal peptide, xynA | This work |
pEC-XK99E | E. coli–C. glutamicum shuttle vector, Kmr | Lab stock |
pEC-XK99E-xynA (pEC-X) | pEC-XK99E derivative, PAH6, cspB2 signal peptide, xynA | This work |
pK18mobSacB | SacB, suicide vector, Kmr | Lab stock |
pK18mobSacB-cspB2 | pK18mobSacB derivative, for cspB2 disruption | This work |
pK18mobSacB-xynA | pK18mobSacB derivative, for xynA integration | This work |