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Table 1 Bacterial strains and plasmids used in this study

From: Development of a secretory expression system with high compatibility between expression elements and an optimized host for endoxylanase production in Corynebacterium glutamicum

Strain or plasmid Description Sources
Strains
 E. coli DH5α   Lab stock
 C. glutamicum ATCC13032 Wild type Lab stock
 C. glutamicum CGMCCl.15647 Wild type Lab stock
 ΔcspB2 C. glutamicum CGMCCl.15647, cspB2 disruption This work
 ΔclpS C. glutamicum CGMCCl.15647, clpS disruption Lab stock
 ΔcspB2ΔclpS C. glutamicum CGMCCl.15647, cspB2 and clpS disruption This work
 ΔcspB2InX C. glutamicum CGMCCl.15647, cspB2 disruption, xynA integration This work
 ΔcspB2ΔclpSInX C. glutamicum CGMCCl.15647, cspB2 disruption, clpS disruption, xynA integration This work
 Cg47+P19-0 C. glutamicum CGMCCl.15647 harboring pXMJ19 This work
 Cg47+P19-X C. glutamicum CGMCCl.15647 harboring pXMJ19-xynA This work
 Cg47+P19-cspB1X C. glutamicum CGMCCl.15647 harboring pXMJ19-cspB1-cspA-xynA This work
 Cg32+P19-X C. glutamicum ATCC13032 harboring pXMJ19-xynA This work
 ΔcspB2+P19-X ΔcspB2 harboring pXMJ19-xynA This work
 ΔclpS+P19-X ΔclpS harboring pXMJ19-xynA This work
 ΔcspB2ΔclpS+P19-X ΔcspB2ΔclpS harboring pXMJ19-xynA This work
 ΔcspB2InX+P19-X ΔcspB2InX harboring pXMJ19-xynA This work
 ΔcspB2ΔclpSInX+P19-X ΔcspB2ΔclpSInX harboring pXMJ19-xynA This work
 ΔcspB2ΔclpSInX+P19-X+pEC-X ΔcspB2ΔclpSInX harboring pXMJ19-xynA and pEC-XK99E-xynA This work
Plasmids
 pXMJ19 (P19-0) E. coliC. glutamicum shuttle vector, Chlr Lab stock
 pXMJ19-cspB2 (P19-cspB2) pXMJ19 derivative, PcspB2, cspB2 signal peptide, cspB2 gene  
 pXMJ19-cspB1-cspA-xynA (P19-cspB1X) PcspB1, cspA signal peptide, xynA This work
 pXMJ19-xynA (P19-X) pXMJ19 derivative, PcspB2, cspB2 signal peptide, xynA This work
 pEC-XK99E E. coliC. glutamicum shuttle vector, Kmr Lab stock
 pEC-XK99E-xynA (pEC-X) pEC-XK99E derivative, PAH6, cspB2 signal peptide, xynA This work
 pK18mobSacB SacB, suicide vector, Kmr Lab stock
 pK18mobSacB-cspB2 pK18mobSacB derivative, for cspB2 disruption This work
 pK18mobSacB-xynA pK18mobSacB derivative, for xynA integration This work