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Table 3 Specific production (μg/gCDW) of different γ-lactones by engineered A. gossypii strains

From: Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones

γ-Lactones

ATCC

DES589

elo624Δ

DES589/elo624Δ

YlPOX2

DES589/elo624Δ/YlPOX2

γ-Octalactone

(1.3 ± 0.2)a × 102

(1.1 ± 0.1)a × 102

(3.2 ± 1.0)d × 101

γ-Nonalactone

6 ± 2

3 ± 0

γ-Decalactone

(1.2 ± 0.1)a × 103

(7.4 ± 0.8)e × 103

98.0 ± 0.2 a

(2.9 ± 0.3)c × 103

(4.9 ± 0.5)e × 103

97.7 ± 0.2 a

(8.8 ± 2.9)a × 102

(5.0 ± 0.5)e × 103

99.3 ± 0.1 e

γ-Undecalactone

3 ± 1

2 ± 0

2 ± 1

γ-Dodecalactone

3 ± 1a

(1.4 ± 0.3)b × 101

2 ± 0a

3 ± 0a

3 ± 1a

2 ± 1a

  1. Underlined values represent the percentage (%) of γ-decalactone regarding the total γ-lactones produced by different high-producing engineered strains. Data are presented as mean ± standard deviation of three biological replicates. Superscript letters represent significant differences to the control (ATCC) between values of the same column. Superscript letters of underlined values represent significant differences between relative productions. a,b(p < 0.05), a,c(p < 0.01), a,d(p < 0.001) and a,e(p < 0.0001)
  2. (–), not detected and considered 0 for the statistical tests