Table 1 Strains and plasmids used in this study
From: Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum
Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
Strain | ||
 E. coli Top10 | General cloning host | Biomed |
 C. glutamicum ATCC 13032 | Wild-type strain | ATCC |
 C. glutamicum Δupp | ATCC 13032 derivative with its upp gene deleted | This study |
 C. glutamicum ΔcrtYe/f | ATCC 13032 derivative with its crtYe/f gene deleted | This study |
 ZJ1 | ATCC 13032 derivative with its pyc gene deleted | This study |
 ZJ2 | ATCC 13032 derivative with its ldh gene deleted | This study |
 ZJ3 | ATCC 13032 derivative with its adhA gene deleted | This study |
 ZJ12 | ATCC 13032 derivative with its pyc gene and ldh gene deleted | This study |
 ZJ13 | ATCC 13032 derivative with its pyc gene and adhA gene deleted | This study |
 ZJ23 | ATCC 13032 derivative with its ldh gene and adhA gene deleted | This study |
 ZJ123 | ATCC 13032 derivative with its pyc gene, ldh gene and adhA gene deleted | This study |
 WT-0 | C. glutamicum ATCC 13032 + pKJ0 | This study |
 WT-1 | C. glutamicum ATCC 13032 + pKJ1 | This study |
 ZJ1-1 | ZJ1 + pKJ1 | This study |
 ZJ2-1 | ZJ2 + pKJ1 | This study |
 ZJ3-1 | ZJ3 + pKJ1 | This study |
 ZJ12-1 | ZJ12 + pKJ1 | This study |
 ZJ13-1 | ZJ13 + pKJ1 | This study |
 ZJ23-1 | ZJ23 + pKJ1 | This study |
 ZJ123-1 | ZJ123 + pKJ1 | This study |
Plasmidb | ||
 pJYS3_ΔcrtYf | pBL1ts ori V C.glutamicum. KanR pSC101 ori V E. coli PlacM-FnCpf1, Pj23119-crRNA targeting crtYf, 1 kb upstream and downstream homologous arms flaking the 705 bp deletion fragment inside crtYf | Jiang et al. [12] |
 pYJ1 | pBLts ori V C.glutamicum KanR p15A ori V E.coli PlacM-FnCpf1, crRNA expression cassette | This study |
 pYJ1_S_Δupp | pYJ1 carrying spacer sequence targeting upp gene | This study |
 pYJ1_SH_Δupp | pYJ1_S_Δupp carrying upstream and downstream homologous arm targeting upp gene | This study |
 pYJ1_S_ΔcrtYe/f | pYJ1 carrying spacer sequence targeting crtYe/f gene | This study |
 pYJ1_SH_ΔcrtYe/f | pYJ1_S_ΔcrtYe/f carrying upstream and downstream homologous arm targeting crtYe/f gene | This study |
 pYJ1_S_Δpyc | pYJ1 carrying spacer sequence targeting pyc gene | This study |
 pYJ1_SH_Δpyc | pYJ1 carrying spacer sequence and homologous arm targeting pyc gene | This study |
 pYJ1_S_Δldh | pYJ1 carrying spacer sequence targeting ldh gene | This study |
 pYJ1_SH_Δldh | pYJ1 carrying spacer sequence and homologous arm targeting ldh gene | This study |
 pYJ1_S_ΔadhA | pYJ1 carrying spacer sequence targeting adhA gene | This study |
 pYJ1_SH_ΔadhA | pYJ1 carrying spacer sequence and homologous arm targeting adhA gene | This study |
 pJET_HA_Δupp | pJET vector carrying upstream and downstream homologous arm targeting upp gene | This study |
 pJET_HA_ΔcrtYe/f | pJET vector carrying upstream and downstream homologous arm targeting crtYe/f gene | This study |
 pKS167 | NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-adhA | Smith et al. [44] |
 pKJ0 | NG2 ori; KanR; Peftu | This study |
 pKJ1 | NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-feaB | This study |