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Table 1 Strains and plasmids used in this study

From: Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum

Strain or plasmid Descriptiona Reference or source
Strain
 E. coli Top10 General cloning host Biomed
 C. glutamicum ATCC 13032 Wild-type strain ATCC
 C. glutamicum Δupp ATCC 13032 derivative with its upp gene deleted This study
 C. glutamicum ΔcrtYe/f ATCC 13032 derivative with its crtYe/f gene deleted This study
 ZJ1 ATCC 13032 derivative with its pyc gene deleted This study
 ZJ2 ATCC 13032 derivative with its ldh gene deleted This study
 ZJ3 ATCC 13032 derivative with its adhA gene deleted This study
 ZJ12 ATCC 13032 derivative with its pyc gene and ldh gene deleted This study
 ZJ13 ATCC 13032 derivative with its pyc gene and adhA gene deleted This study
 ZJ23 ATCC 13032 derivative with its ldh gene and adhA gene deleted This study
 ZJ123 ATCC 13032 derivative with its pyc gene, ldh gene and adhA gene deleted This study
 WT-0 C. glutamicum ATCC 13032 + pKJ0 This study
 WT-1 C. glutamicum ATCC 13032 + pKJ1 This study
 ZJ1-1 ZJ1 + pKJ1 This study
 ZJ2-1 ZJ2 + pKJ1 This study
 ZJ3-1 ZJ3 + pKJ1 This study
 ZJ12-1 ZJ12 + pKJ1 This study
 ZJ13-1 ZJ13 + pKJ1 This study
 ZJ23-1 ZJ23 + pKJ1 This study
 ZJ123-1 ZJ123 + pKJ1 This study
Plasmidb
 pJYS3_ΔcrtYf pBL1ts ori V C.glutamicum. KanR pSC101 ori V E. coli PlacM-FnCpf1, Pj23119-crRNA targeting crtYf, 1 kb upstream and downstream homologous arms flaking the 705 bp deletion fragment inside crtYf Jiang et al. [12]
 pYJ1 pBLts ori V C.glutamicum KanR p15A ori V E.coli PlacM-FnCpf1, crRNA expression cassette This study
 pYJ1_S_Δupp pYJ1 carrying spacer sequence targeting upp gene This study
 pYJ1_SH_Δupp pYJ1_S_Δupp carrying upstream and downstream homologous arm targeting upp gene This study
 pYJ1_S_ΔcrtYe/f pYJ1 carrying spacer sequence targeting crtYe/f gene This study
 pYJ1_SH_ΔcrtYe/f pYJ1_S_ΔcrtYe/f carrying upstream and downstream homologous arm targeting crtYe/f gene This study
 pYJ1_S_Δpyc pYJ1 carrying spacer sequence targeting pyc gene This study
 pYJ1_SH_Δpyc pYJ1 carrying spacer sequence and homologous arm targeting pyc gene This study
 pYJ1_S_Δldh pYJ1 carrying spacer sequence targeting ldh gene This study
 pYJ1_SH_Δldh pYJ1 carrying spacer sequence and homologous arm targeting ldh gene This study
 pYJ1_S_ΔadhA pYJ1 carrying spacer sequence targeting adhA gene This study
 pYJ1_SH_ΔadhA pYJ1 carrying spacer sequence and homologous arm targeting adhA gene This study
 pJET_HA_Δupp pJET vector carrying upstream and downstream homologous arm targeting upp gene This study
 pJET_HA_ΔcrtYe/f pJET vector carrying upstream and downstream homologous arm targeting crtYe/f gene This study
 pKS167 NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-adhA Smith et al. [44]
 pKJ0 NG2 ori; KanR; Peftu This study
 pKJ1 NG2 ori; KanR; Peftu::alsS-ilvCD-kivd-feaB This study
  1. aKanR represents resistance to kanamycin
  2. bcrRNA expression plasmids with changed spacer sequences are not listed here. A full list of spacer sequences used in this study is shown in Additional file 1: Table S2