Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool

Ziko, Laila; Saqr, Al-Hussein A.; Ouf, Amged; Gimpel, Matthias; Aziz, Ramy K.; Neubauer, Peter; Siam, Rania

doi:10.1186/s12934-019-1103-3

Microbial Cell Factories

Table 2 Annotation of selected PEGs of putative orphan biosynthetic gene clusters

From: Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool

Proposed functions of cluster elements	Scaffold	Start	Stop	PEG	RAST annotation	Best hit from PSI-BLAST	Query coverage %	E-value	Identity %	Accession number
(a) 14-7E selected PEGs annotation
Regulatory gene	Scaffold_C361	395	1345	28	Transcription initiation factor B	Transcription initiation factor IIB [candidate division MSBL1 archaeon SCGC-AAA259D14]	95	3e−138	65	gi\|985656859\|KXA89394.1
Biosynthesis of specialized metabolite/peptide modification	Scaffold_C371	2077	3141	54	Dolichol-phosphate mannosyltransferase (EC 2.4.1.83) homolog	Dolichol monophosphate mannose synthase [Chloroflexi bacterium CG08_land_8_20_14_0_20_45_12]	99	9e−109	47	gi\|1277809164\|PIU23225.1
	Scaffold_C383	437	1396	73	Radical SAM domain protein	Radical SAM/SPASM domain-containing protein [Thermodesulfobium narugense]	83	2e−18	28	gi\|503521935\|WP_013756246.1
	Scaffold_16	18334	17825	202	Geranylgeranyl diphosphate synthase (EC 2.5.1.29)	Hypothetical protein AKJ41_03425 [candidate division MSBL1 archaeon SCGC-AAA259O05]	100	8e−80	75	gi\|985669946\|KXB00917.1
Subtilases	Scaffold_16	11832	13133	200	Peptidase S8 and S53, subtilisin, kexin, sedolisin	Subtilase family protein [Micromonospora cremea]	37	1e−11	33	gi\|1118770207\|SIM81338.1
Subtilases	Scaffold_3	3824	3937	232	Hypothetical protein	Peptidase S8/S53 subtilisin kexin sedolisin [Halothermothrix orenii]	82	8e−14	35	gi\|501769773\|WP_012635463.1
Resistance to specialized metabolite	Scaffold_3	3169	3348	231	Hypothetical protein	ABC transporter permease [Desulfitobacterium sp. PCE1]	86	0.076*	32	gi\|518688679\|WP_019850372.1
	Scaffold_2	9816	8962	218	NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)	Glyceraldehyde-3-phosphate dehydrogenase [candidate division MSBL1 archaeon SCGC-AAA261F17]	99	4e−166	83	gi\|985671588\|KXB02384.1
	Scaffold_2	9970	9818	219	NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)	Glyceraldehyde-3-phosphate dehydrogenase [candidate division MSBL1 archaeon SCGC-AAA261F17]	92	2e−18	89	gi\|985671588\|KXB02384.1
(b) 10-2G selected PEGs annotation
Synthesis of non-ribosomal peptide	Scaffold_7	29	160	20	Hypothetical protein	Non-ribosomal peptide synthetase [Mycobacterium minnesotense]	67	0.18*	45	gi\|1178501113\|WP_083022343.1
Biosynthesis of specialized metabolite/peptide modification	Scaffold_7	3719	4762	29	Glycosyltransferase	Glycosyltransferase Family 4 protein [Enhydrobacter aerosaccus]	98	1e−30	28	gi\|1194598116\|WP_085933361.1
	Scaffold_3	202	702	3	Hypothetical protein	Class I SAM-dependent methyltransferase [Methanosarcina mazei]	78	4e−12	34	gi\|850504616\|WP_048046626.1
	Scaffold_3	2297	2989	6	Glycosyltransferase	Glycosyl transferase [Parcubacteria group bacterium CG11_big_fil_rev_8_21_14_0_20_39_14]	77	5e−35	38	gi\|1277086632\|PIQ92058.1
Resistance to specialized metabolite	Scaffold_3	2961	3542	7	Hypothetical protein	ABC transporter permease [Hymenobacter sp. CRA2]	47	0.73*	27	gi\|1150819492\|WP_078012825.1

Annotation of the putative biosynthetic gene cluster elements in: (a) 14-7E and (b) 10-2G. * PEGs with PSI-BLAST hits of E-value > 0.005. The proposed functions of the biosynthetic gene cluster elements are denoted, each function beside its relevant annotated gene. The scaffold number is denoted, as well as the start, stop and PEG number. RAST annotation for each gene is then denoted. Lastly, PSI-BLAST best hit for each gene is denoted, its query coverage, percentage identity and best hit accession number

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ISSN: 1475-2859

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