Table 1 Assembly metrics of the bioactive fosmid insert DNA from Red Sea ATII LCL
Assembly statistics | 14-7E insert | 10-2G insert |
|---|---|---|
Sequencing method | Illumina HiSeq: TruSeq DNA PCR Free (350) | Illumina MiSeq: 300 bp paired—end read (Illumina MiSeq V3) |
De novo assembly program | SOAPdenovo2 | CLC Genomics Workbench v 8.0 |
Total bases | 2,044,687,834 | 72,217,200 |
Total reads | 20,244,434 | 240,724 |
Total reads after quality filtering | 14,876,068 | 240,658 |
Total bases in scaffolds | 201,086 | 2,452,525 |
Scaffold N50 | 15,392 | 1891 |
Number of scaffolds | 29 | 1318 |
Total bases in scaffolds after filtering and trimming | 201,086 | 21,407 |
Scaffold N50 after filtering and trimming | 15,392 | 11,374 |
Number of scaffolds after filtering and trimming | 29 | 14 |
Number of scaffolds with RAST-annotated PEGs | 27 | 2 |
Number of PEGs | 289 | 30 |