Table 1 List of strains constructed and used in this study

CEN.PK 113-5D (LW00)

MATa MAL2-8c SUC2 ura3-52

–

P. Kötter (University of Frankfurt, Germany)

LW01

MATa MAL2-8c SUC2 ura3-52 elo3Δ

–

Wenning et al. [21]

LW02

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

–

This study

LW03

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

–

This study

LW04

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212

This study

LW05

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::ELO2::OLE1

This study

LW06

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::AtFAE::OLE1

This study

LW07

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::BnKCS::OLE1

This study

LW08

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::CaKCS::OLE1

This study

LW09

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::LaKCS::OLE1

This study

LW10

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::SciFAE::OLE1

This study

LW11

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::TmKCS::OLE1

This study

LW12

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::CaKCS::SciFAD-SP

This study

LW13

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::CaKCS::ChDes9-1

This study

LW14

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::CaKCS::AtADS1.2

This study

LW15

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::CaKCS::AtADS1.4

This study

LW16

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::LaKCS::SciFAD-SP

This study

LW17

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::LaKCS::ChDes9-1

This study

LW18

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::LaKCS::AtADS1.2

This study

LW19

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

pYX212::LaKCS::AtADS1.4

This study

LW20

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

XI-5::cas9-URA

–

This study

LW21

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

XI-5::cas9-URA

X-4::FAA1::ELO1

This study

LW22

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

XI-5::cas9-URA

X-4::FAA1::ELO1

XII-5::CaKCS::OLE1

This study

LW23

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

XI-5::cas9

X-4::FAA1::ELO1

pYX212::MaFAldhR::SciWS::ELO2

This study

LW24

MATa MAL2-8c SUC2 ura3-52 elo3Δ

X-2::pMPC3::ACC1**

X-3::IFA38::PHS1::TSC13::ACB1

XI-5::cas9

X-4::FAA1::ELO1

XII-5::CaKCS::OLE1

pYX212::MaFAldhR::SciWS::ELO2

This study