Table 1 List of primers used in this study
From: Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules
Name | Description | Oligo sequence (5–3′) |
|---|---|---|
ab156 | chnR/pChnB, PstI | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGATTACGACATGTGAATTTATTCAAAATCTGC |
ab155 | chnR/pChnB, EcoRI | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCTAGGGCGGCGGATTTGTCC |
ab152 | pBAV1C rev. | TCATGAATCAAAGGACGCTATTG |
ab151 | pBAV1C for. | GTCAAATATTCATAAGAACCTTTGATATAATC |
GFP fwd | GFP | TGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGCGTAAAGGTGAAGAACTGTTCAC |
GFP rev | GFP | TAATACTGCAGTTAAGCTACTAAAGCGTAGTTTTCGTCGTTTGCAGCAGGCCTTTTGTAGAGTTCATCCATGCCGTG |
vs15_2 44 | undA, PstI | TAATCTGCAGCGGCCGCTACTAGTATTATCAGCCCGCAGCCAAC |
vs15_1 68 | undA, XbaI | TAATGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATTGACGCATTTGTTCGTATC |
tl15 | ‘tesA, XbaI | TGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCGGACACGTTATTGATTCTGGG |
tl16 | ‘tesA, PstI | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATGAGTCATGATTTACTAAAGGCTGC |