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Table 2 Bacterial strains and plasmids used in this work

From: Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system

Name Relevant characteristics Source or reference
DH10B-T1R (F λ) mcrA Δmrr-hsdRMS-mcrBC φ80lacZΔM15, ΔlacX74, recA1, endA1, Δ(ara, leu)7697 galU galK rpsL(StrR), nupG tonA Novagen-Merck
HB2151 Δlac-pro, ara, nalR, thi, F’(proAB lacIQ lacZΔM15) [68]
TG1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rKmK−) thi-1 supE [F´ traD36 proAB lacIqZΔM15] Stratagene-ThermoFisher
BL21 (DE3) F; ompT hsdSB(rB−, mB−) gal dcm lon λ(DE3[lacI lacUV5-T7 gene1 ind1 sam7 nin5]) Novagen-Merck
pCANTAB-5Ehis ApR; phagemid vector for periplasmic production of Nb-pIII fusions with His and E-tag [31]
pEHlyA5 ApR; pUC ori, lac promoter, N-terminal His tag, VHH, HA and E-tags, C-HlyA This work
pVDL9.3 CmR; expression of HlyB and HlyD [55]
pET28a KmR; pBR ori, T7 promoter, N-terminal His-tagged fusions Novagen-Merck
pET28a-TirMEHEC pET28a derivative; expression of His-tagged TirM of EHEC This work
pET28a-Int280EHEC pET28a derivative; expression of His-tagged Int280 of EHEC This work
pET28a-EspAEHEC pET28a derivative; expression His-tagged EspA of EHEC This work