Table 2 Bacterial strains and plasmids used in this work

DH10B-T1R

(F⁻ λ⁻) mcrA Δmrr-hsdRMS-mcrBC φ80lacZΔM15, ΔlacX74, recA1, endA1, Δ(ara, leu)7697 galU galK rpsL(Str^R), nupG tonA

Novagen-Merck

HB2151

Δlac-pro, ara, nal^R, thi, F’(proAB lacI^Q lacZΔM15)

[68]

TG1

Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK− mK−) thi-1 supE [F´ traD36 proAB lacIqZΔM15]

Stratagene-ThermoFisher

BL21 (DE3)

F⁻; ompT hsdSB(rB−, mB−) gal dcm lon λ(DE3[lacI lacUV5-T7 gene1 ind1 sam7 nin5])

Novagen-Merck

pCANTAB-5Ehis

Ap^R; phagemid vector for periplasmic production of Nb-pIII fusions with His and E-tag

[31]

pEHlyA5

Ap^R; pUC ori, lac promoter, N-terminal His tag, VHH, HA and E-tags, C-HlyA

This work

pVDL9.3

Cm^R; expression of HlyB and HlyD

[55]

pET28a

Km^R; pBR ori, T7 promoter, N-terminal His-tagged fusions

Novagen-Merck

pET28a-TirM_EHEC

pET28a derivative; expression of His-tagged TirM of EHEC

This work

pET28a-Int280_EHEC

pET28a derivative; expression of His-tagged Int280 of EHEC

This work

pET28a-EspA_EHEC

pET28a derivative; expression His-tagged EspA of EHEC

This work