Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides

Table 2 Genes modified in this study

Gene name	KEGG ID	Annotated enzyme	Gene modification
phaA1	RSP_0745	Acetyl-CoA acetyltransferase	Over-expression
phaA2	RSP_1354	Acetyl-CoA acetyltransferase	Over-expression
phaA3	RSP_2197	Acetyl-CoA acetyltransferase	Over-expression
phaA4	RSP_3184	Acetyl-CoA acetyltransferase	Over-expression
phaB1	RSP_0747	Acetoacetyl-CoA reductase	Over-expression
phaB2	RSP_3963	Acetoacetyl-CoA reductase	Over-expression
phaC1	RSP_0382	Poly (3-hydroxyalkanoate) polymerase	Over-expression
phaC2	RSP_1257	Poly (3-hydroxyalkanoate) polymerase	Over-expression
phaZ	RSP_0383	Poly (3-hydroxyalkanoate) depolymerase	Disruption

ISSN: 1475-2859