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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis

Fig. 3

Identification of the ΔSSP_Lrp and ΔSSP_Lrp-SP mutant. a The SSP_Lrp gene inactivated by insertion of the aparamycin resistance cassette, 1: S. spiramyceticus 1941, 2–6: ΔSSP_Lrp; b design of the SSP_Lrp deletion (shown in red) in the C-terminal. c Confirmation of ΔSSP_Lrp-SP by PCR and sequencing. 1: S. spiramyceticus 1941; 2: ΔSSP_Lrp-SP mutant

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