Table 2 Primers used in this work and EcoRI and NotI sites were lowercase italic
Primers | Sequence (5′–3′) | Purpose |
|---|---|---|
SsAET-F | CGACGGgaattcATGAAAAATACAATTTCGTGCC | SsAET clone |
SsAET-R | ATAAGAATgcggccgcCTAATGGTGATGGTGATGATGATCTTTGAGGACAGAAAATTCG | SsAET clone |
GS115-F | CAGCTTTGATGCCTGAAATC | PCR verification for both |
GPA-R | GCATCCTGAAGAAACAATACG | PCR verification for GPA |
GPAp-R | CATTCCAGAATTGAACGGAG | PCR verification for GPAp |
AOX1-F | CTGGTTCCAATTGACAAGC | DNA sequencing for both |
AOX1-R | TGGCATTCTGACATCCTC | DNA sequencing for both |