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Table 4 Results of chemical analysis of products prepared from fermentation broth using optimal agitation and static processes

From: Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory

Substrates/products TUDCA
(%)
TCDCA
(%)
T-7-KLCA
(%)
TUCA
(%)
TCA
(%)
UDCA
(%)
TUDCA + TCDCA (%) Five bile acids (%) Moisture
(%)
Endotoxin
(EU/mL)
Weight
(g)
Refine chicken bile powder (RCBP)a 0.00 65.00 0.00 0.00 7.40 0.40 65.00 72.80 2.8 ± 0.2 0.001 30.00
Products from RCBPb 35.85 ± 3.85 24.10 ± 2.65 3.54 ± 0.48 8.69 ± 2.75 2.45 ± 0.49 0.22 ± 0.08 58.91 ± 5.77 74.85 ± 6.94 4.68 ± 1.11 0.003 ± 0.001 29.35 ± 2.83
Crude chicken bile powder (CCBP)a 0.00 45.00 0.00 0.00 13.00 0.30 45.00 58.30 4.1 ± 0.4 0.0010 35.00
Products from CCBPb 27.14 ± 4.23 17.48 ± 1.68 4.29 ± 0.54 13.06 ± 1.20 2.37 ± 0.15 2.44 ± 0.30 43.91 ± 5.33 66.79 ± 6.96 2.26 ± 0.28 0.003 ± 0.000 30.78 ± 3.04
  1. aData of refine chicken bile powder and crude chicken bile powder were separately from one batch
  2. bData of products from RCBP and CCBP were from three batches, respectively