Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory

Table 4 Results of chemical analysis of products prepared from fermentation broth using optimal agitation and static processes

Substrates/products	TUDCA (%)	TCDCA (%)	T-7-KLCA (%)	TUCA (%)	TCA (%)	UDCA (%)	TUDCA + TCDCA (%)	Five bile acids (%)	Moisture (%)	Endotoxin (EU/mL)	Weight (g)
Refine chicken bile powder (RCBP)^a	0.00	65.00	0.00	0.00	7.40	0.40	65.00	72.80	2.8 ± 0.2	0.001	30.00
Products from RCBP^b	35.85 ± 3.85	24.10 ± 2.65	3.54 ± 0.48	8.69 ± 2.75	2.45 ± 0.49	0.22 ± 0.08	58.91 ± 5.77	74.85 ± 6.94	4.68 ± 1.11	0.003 ± 0.001	29.35 ± 2.83
Crude chicken bile powder (CCBP)^a	0.00	45.00	0.00	0.00	13.00	0.30	45.00	58.30	4.1 ± 0.4	0.0010	35.00
Products from CCBP^b	27.14 ± 4.23	17.48 ± 1.68	4.29 ± 0.54	13.06 ± 1.20	2.37 ± 0.15	2.44 ± 0.30	43.91 ± 5.33	66.79 ± 6.96	2.26 ± 0.28	0.003 ± 0.000	30.78 ± 3.04

^aData of refine chicken bile powder and crude chicken bile powder were separately from one batch
^bData of products from RCBP and CCBP were from three batches, respectively

ISSN: 1475-2859