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Table 2 Solutions given by Box–Behnken Design for medium composition

From: Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory

Solution no.

Suggested concentration (g/L)

Expected

Desirability

Glucose

Glycerol

Tryptone

Yeast extract

OD600

Conversion efficiency

1

0.00

28.43

0.00

20.00

7.45

22.7%

0.23

2

0.01

27.87

0.00

20.00

7.44

22.7%

0.23

3

0.00

26.99

0.00

20.0

7.41

22.65

0.23

4

0.00

30.00

0.00

19.37

7.55

22.45

0.22

5

0.00

24.17

0.00

19.98

7.36

22.44

0.22

6

0.17

29.84

0.01

20.0

7.57

22.30

0.22

7

0.20

30.00

0.00

20.0

7.58

22.23

0.22

8

0.00

30.00

0.00

18.70

7.60

22.13

0.22

9

0.00

21.04

0.04

19.97

7.34

22.09

0.22

10

0.00

30.00

2.40

20.00

7.42

22.00

0.22

  1. The top 10 of 55 solutions were listed and the selected one was highlighted in italics