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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

Fig. 3

Accumulation of the scFv construct and its misfolded variant in CyDisCo cells. TorA-scFv and the misfolded TorA-scFv constructs were expressed in E. coli cells together with Erv1p and PDI, CyDisCo components. After expression, cells were fractionated into cytoplasmic (C), membrane (M), periplasmic (P) and inclusion body (IB) samples and extracted proteins separated via SDS-PAGE

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