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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture

Fig. 4

FFA productivity, FFA composition, and growth of Rs-A2, Rs-CA2, Rs-DCA2, Rs-AccCA2, and Rs-HCA2. a The recombinant R. sphaeroides strains were grown photoheterotrophically in Sis minimal medium and harvested at the exponential growth phase (OD660 of 2.5, 2.3, 2.3, 2.4, 2.3 and 2.2 for WT, Rs-A2, Rs-CA2, Rs-DCA2, Rs-AccCA2 and Rs-HCA2, respectively). FFAs in the culture supernatant were determined, and the FFA productivity is shown with error bars from three independent experiments. b The composition of FFAs is illustrated as the percentage of the total moles of FFAs. The experiments were independently repeated three times; the data shown are for one of three representative experiments. c Doubling times of recombinant R. sphaeroides strains are shown with WT cells as a control. The data presented here were reproduced within SDs of 10–15%. The experiments were independently repeated three times, and the data shown are for one of three representative experiments

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