Fig. 1
From: Biosensor-guided improvements in salicylate production by recombinant Escherichia coli
Sensor-based pathway optimization for improving salicylate production. a Synthetic pathway for producing salicylate from glycerol. Six genes highlighted in blue encode the key enzymes involved in salicylate biosynthesis: ppsA encodes PEP synthetase, which converts pyruvate to PEP. tktA encodes a transketolase that increases supply of E4P. DAHP synthase encoded by aroGfbr catalyzes the aldol condensation to generate DAHP. Shikimate kinase encoded by aroL produces chorismate. Isochorismate synthase (encoded by entC) and isochorismate pyruvate lyase (encoded by pchB) enable the synthesis of salicylate form chorismate. b AraC-based salicylate biosensor was used to screen for clones showing enhanced salicylate production