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Fig. 2 | Microbial Cell Factories

Fig. 2

From: High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Fig. 2

Comparison of rhFGF21 gene expression with different promoters. a SDS-PAGE analysis of rhFGF21 expressed in the cytoplasm of B. subtilis following 24 h incubation. The molecular weight of mature rhFGF21 is about 27 kDa. Column M: molecular weight marker. The strain B. subtilis 1A751C served as negative control. b The relative densitometric analysis for rhFGF21 expression level in different B. subtilis strains

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