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Table 3 Primers used in this work

From: Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440

Primers

Sequences 5′–3′

N46S F

TGGTCTGCGTTTACTGAGCACAGTTGCCCACAATG

V48G F

GCGTTTACTGAATACAGGTGCCCACAATGCCGTGA

V48Q F

GCGTTTACTGAATACACAGGCCCACAATGCCGTGA

M186L F

GATTCGCGCAGTGACACTGAGCTTCAGCCCGGGTC

M186G F

GATTCGCGCAGTGACAGGTAGCTTCAGCCCGGGTC

L269G F

CAGTTCTTTAGACATCGGTCAAGGTGGCGTTGTGC

L269V F

CAGTTCTTTAGACATCGTTCAAGGTGGCGTTGTGC

D305A F

CGTTGATCCGGTTCTGGCCCAAGGTGCCAATATGG

D305X F

CGTTGATCCGGTTCTGNNNCAAGGTGCCAATATGG

R

GCCTTACTGGTTAGCAGAATG

  1. The bold type shows the site of the variations. Saturated mutation sites were substituted by bold and italic NNN (N = A/G/C/T). In addition, all the site of variations share the same downstream primer
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Microbial Cell Factories

ISSN: 1475-2859

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