Fig. 7
Biotransformation of styrene to (S)-styrene oxide by whole cells of recombinant Pseudomonas putida KT2440 in different solvents. The whole cell biotransformation was conducted by Pseudomonas putida KT2440/pJB861-styABD305G-fdh toward styrene. The reaction was carried out in 50 mL flasks with 10 mL of KP buffer (200 mM, pH 8.0) containing 26 mM of the substrate styrene and biomass with a cell dry weight of 1.0 g, incubated at 30 °C for 8 h. And the reaction system was substituted with different water-miscible (10%, v/v) and water-immiscible (1:1, v/v) solvents. The product was withdrawn periodically and analyzed by reverse phase HPLC on a Luna C18 column at a flow rate of 0.8 mL/min under a methanol–water mixture at a ratio of 75:25. The reaction mixture after 15 min was used to determine specific epoxidation activities. All assays were performed in triplicate; each column represents the mean of triplicate assays