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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus

Fig. 2

Identification of recombinant proteins and characterization of their stability via Western blotting analysis. a Immunoblot analysis of total whole-cell protein extracts from recombinant r-L. lactis-OptiVP2-RCK (lane 1), r-L. lactis-OptiVP2 (lane 2) and wt-L. lactis (lane 3). Proteins were separated on 12% SDS polyacrylamide gels and reacted with a VP2 Mab. b Detection of VP2-RCK fusion protein expression from recombinant r-L. lactis-OptiVP2 every 24 h for a total of 120 h via western blotting analysis (lanes 1–5). Sizes of the protein molecular mass standards are indicated to the left or right of each blot. c Western immunoblot analysis localization of VP2-RCK protein in recombinant r-L. lactis-OptiVP2-RCK cells. The cell surface fraction of r-L. lactis-OptiVP2-RCK (lane 1), culture supernatant of r-L. lactis-OptiVP2-RCK (lane 2), supernatant of ultrasonically lysed r-L. lactis-OptiVP2-RCK (lane 3), precipitate of ultrasonically lysed r-L. lactis-OptiVP2-RCK (lane 4). The sizes of molecular mass protein standards are indicated to the right

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Microbial Cell Factories

ISSN: 1475-2859

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