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Table 3 Secondary structure of rPstS-1 from P. pastoris and E. coli. Comparative secondary structure content obtained by CD data analysis, NMR data and CAPITO server

From: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

  NMR [94] CAPITO (CD)
  rPstS-1 from E. coli rPstS-1 from P. pastoris
PDB: 1PC3 125 μg/mL 250 μg/mL 125 μg/mL 250 μg/mL
Helix (%) 34 16 20 10 5
β-strand (%) 18 28 29 38 40
Irregular (%) 41 50 52 56 53
  1. CAPITO CD Analysis & Plotting Tool [93], CD circular dichroism, NMR, nuclear magnetic resonance; PDB protein data bank