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Table 3 Secondary structure of rPstS-1 from P. pastoris and E. coli. Comparative secondary structure content obtained by CD data analysis, NMR data and CAPITO server

From: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

 

NMR [94]

CAPITO (CD)

 

rPstS-1 from E. coli

rPstS-1 from P. pastoris

PDB: 1PC3

125 μg/mL

250 μg/mL

125 μg/mL

250 μg/mL

Helix (%)

34

16

20

10

5

β-strand (%)

18

28

29

38

40

Irregular (%)

41

50

52

56

53

  1. CAPITO CD Analysis & Plotting Tool [93], CD circular dichroism, NMR, nuclear magnetic resonance; PDB protein data bank