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Table 2 Summary of purification of rPstS-1

From: Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Purification stage Recovery of total protein (mg/L)a rPsts-1 (mg/L)a Recovery % Purity %
Total protein from supernatant 185 ± 9 46 ± 4 100 25 ± 3
Ultrafiltration (> 100 kDa) 80.0 ± 2.9 18.9 ± 5.0 41.0 ± 14.9 23.5 ± 5.4
Ultrafiltration (100–10 kDa) 49.0 ± 3.4 13.2 ± 4.2 28.6 ± 7.1 26.5 ± 6.7
Reverse phase chromatography 4.3 ± 0.2 4.2 ± 0.2 9.1 ± 1.1 97.7 ± 0.6
  1. aTotal supernatant protein was calculated by Bradford method. The rPstS-1 protein was calculated by densitometry in gel after each stage of purification, and each protein purification step was calculated per liter of initial cell culture volume