Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection

Table 2 Summary of purification of rPstS-1

Purification stage	Recovery of total protein (mg/L)^a	rPsts-1 (mg/L)^a	Recovery %	Purity %
Total protein from supernatant	185 ± 9	46 ± 4	100	25 ± 3
Ultrafiltration (> 100 kDa)	80.0 ± 2.9	18.9 ± 5.0	41.0 ± 14.9	23.5 ± 5.4
Ultrafiltration (100–10 kDa)	49.0 ± 3.4	13.2 ± 4.2	28.6 ± 7.1	26.5 ± 6.7
Reverse phase chromatography	4.3 ± 0.2	4.2 ± 0.2	9.1 ± 1.1	97.7 ± 0.6

^aTotal supernatant protein was calculated by Bradford method. The rPstS-1 protein was calculated by densitometry in gel after each stage of purification, and each protein purification step was calculated per liter of initial cell culture volume

ISSN: 1475-2859