Fig. 9

Secreted titres of a range of substrates through the optimised Mark IV secretion strain. E. coli ΔfliC ΔflgKL ΔclpX ΔmotAB expressing plasmid based SC1 (Mark IV strains), with either cutinase, CH₂, hGH or TrxA cargo, were grown as outlined in Fig. 3 and prepared, along with a relevant protein standard (exception: cutinase, where a hGH standard was utilised), for immunoblot detection with anti-FLAG-HRP (αFLAG). The concentration of secreted protein was then quantified by densitometry analysis. Two biological replicates (with the exception of hGH), ± SE and individual data points shown