Fig. 9
Secreted titres of a range of substrates through the optimised Mark IV secretion strain. E. coli ΔfliC ΔflgKL ΔclpX ΔmotAB expressing plasmid based SC1 (Mark IV strains), with either cutinase, CH2, hGH or TrxA cargo, were grown as outlined in Fig. 3 and prepared, along with a relevant protein standard (exception: cutinase, where a hGH standard was utilised), for immunoblot detection with anti-FLAG-HRP (αFLAG). The concentration of secreted protein was then quantified by densitometry analysis. Two biological replicates (with the exception of hGH), ± SE and individual data points shown
