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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein

Fig. 1

Comparison of secretion capacity in the HAP-less and cap-less strains. E. coli MC1000 ΔfliC ΔflgKL (ΔCKL) and ΔfliCD containing the plasmid pTrc-FliC-ΔD3 were grown in LB supplemented with 0.05 mM IPTG, and harvested at OD600 1.5. Samples which represented either 25 µL or 400 µL culture media were loaded for SDS-PAGE, for intracellular and secreted protein respectively. a Representative images showing the secreted fractions following Coomassie staining, and the intracellular fractions following immunoblotting using an anti-flagellin (H48) antibody and a HRP secondary. A FliC-ΔD3 protein standard (S) was also included, to allow quantification of protein concentration by densitometry (b, c). Quantifications for biological triplicates, ± SE and individual data points shown, *p < 0.05

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