Table 1 The different vectors, plasmids, and strains used in this study
From: Combinatorial approach for improved cyanidin 3-O-glucoside production in Escherichia coli
Vectors and plasmids | Description | Source/references |
|---|---|---|
pGEM®-T Easy | E. coli cloning vector, T7 and SP6 promoters, f1 ori, Ampr | Promega, USA |
pET28 a(+) | E. coli expression vector, f1 pBR322 ori, Kmr | Novagen, Germany |
piBR181 | Multi mono-cistronic vector modified from pET28a+, f1 pBR322 ori, Kmr | Chaudhary et al. [28] |
piBRTrc | Multi mono-cistronic vector modified from pET28a+, f1 pBR322 ori, Kmr | This study |
piBRUV5 | Multi mono-cistronic vector modified from pET28a+, f1 pBR322 ori, Kmr | This study |
piBR181.Apr | piBR181 vector carrying aprr | This study |
piBRTrc.Apr | piBRTrc vector carrying aprr | This study |
piBRUV5.Apr | piBRUV5 vector carrying aprr | This study |
piBR181.gfp | piBR181 vector carrying gfp | This study |
piBRTrc.gfp | piBRTrc vector carrying gfp | This study |
piBRUV5.gfp | piBRUV5 vector carrying gfp | This study |
piBR181-PhANS | piBR181 vector carrying PhANS | This study |
piBR181-At3GT | piBR181 vector carrying At3GT | This study |
piBR181-PhANS.At3GT | piBR181 vector carrying PhANS.At3GT | This study |
piBR181- At3GT. PhANS | piBR181 vector carrying At3GT.PhANS | This study |
piBRTrc-PhANS | piBRTrc vector carrying PhANS | This study |
piBRTrc-At3GT | piBRTrc vector carrying At3GT | This study |
piBRTrc-PhANS.At3GT | piBRTrcvector carrying PhANS.At3GT | This study |
piBRTrc- At3GT. PhANS | piBRTrc vector carrying At3GT.PhANS | This study |
piBRUV5-PhANS | piBRUV5 vector carrying PhANS | This study |
piBRUV5-At3GT | piBRUV5 vector carrying At3GT | This study |
piBRUV5-PhANS.At3GT | piBRUV5 vector carrying PhANS.At3GT | This study |
piBRUV5- At3GT. PhANS | piBRUV5 vector carrying At3GT.PhANS | This study |
pIBR181-glf.glk.pgm2.galU | piBR181 vector carrying glf.glk.pgm2.galU | Parajuli et al. [31] |
piBR181-glf.glk.pgm2.galU.PhANS.At3GT | piBR181 vector carrying glf.glk.pgm2.galU.PhANS.At3GT | This study |
piBR181-glf.glk.pgm2.galU.At3GT.PhANS | piBR181 vector carrying glf.glk.pgm2.galU.At3GT.PhANS | This study |
piBRTrc-glf.glk.pgm2.galU.PhANS.At3GT | piBRTrc vector carrying glf.glk.pgm2.galU under T7 promoter and PhANS.At3GT under trc promoter | This study |
piBRTrc-glf.glk.pgm2.galU.At3GT.PhANS | piBRTrc vector carrying glf.glk.pgm2.galU under T7 promoter and At3GT.PhANS under trc promoter | This study |
piBRUV5-glf.glk.pgm2.galU.PhANS.At3GT | piBRUV5 vector carrying glf.glk.pgm2.galU under T7 promoter and PhANS.At3GT under lacUV5 promoter | This study |
piBRUV5-glf.glk.pgm2.galU.At3GT.PhANS | piBRUV5 vector carrying glf.glk.pgm2.galU under T7 promoter and At3GT.PhANS under lacUV5 promoter | This study |
Strains | Description | Source/references |
|---|---|---|
Escherichia coli XL-1 blue (MRF′) | General cloning host | Stratagene, USA |
E. coli BL21 (DE3) | ompT hsdT hsdS (rB-mB-) gal (DE3) | Novagen, Germany |
E. coli BL21 (DE3) piBR181.Apr | E. coli BL21 (DE3) carrying piBR181.Apr | This study |
E. coli BL21 (DE3) piBRTrc.Apr | E. coli BL21 (DE3) carrying piBRTrc.Apr | This study |
E. coli BL21 (DE3) piBRUV5.Apr | E. coli BL21 (DE3) carrying piBRUV5.Apr | This study |
E. coli BL21 (DE3) piBR181.gfp | E. coli BL21 (DE3) carrying piBR181.gfp | This study |
E. coli BL21 (DE3) piBRTrc.gfp | E. coli BL21 (DE3) carrying piBRTrc.gfp | This study |
E. coli BL21 (DE3) piBRUV5.gfp | E. coli BL21 (DE3) carrying piBRUV5.gfp | This study |
E. coli BL21 (DE3) piBRUV5-PhANS | E. coli BL21 (DE3) carrying piBRUV5-PhANS | This study |
E. coli BL21 (DE3) piBRUV5-At3GT | E. coli BL21 (DE3) carrying piBRUV5-At3GT | This study |
E. coli BL21 (DE3) piBR181-PhANS | E. coli BL21 (DE3) carrying piBR181-PhANS | This study |
E. coli BL21 (DE3) piBR181-At3GT | E. coli BL21 (DE3) carrying piBR181-PhANS | This study |
E. coli BL21 (DE3) piBRTrc-PhANS | E. coli BL21 (DE3) carrying piBRTrc-PhANS | This study |
E. coli BL21 (DE3) piBRTrc-At3GT | E. coli BL21 (DE3) carrying piBRTrc-At3GT | This study |
E. coli BL21 (DE3) piBRUV5-PhANS.At3GT (S1) | E. coli BL21 (DE3) carrying piBRUV5-At3GT.PhANS in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRUV5- At3GT. PhANS (S2) | E. coli BL21 (DE3) carrying piBRUV5-At3GT.PhANS in monocistronic configuration | This study |
E. coli BL21 (DE3) piBR181-PhANS.At3GT (S3) | E. coli BL21 (DE3) carrying piBR181-PhANS.At3GT in monocistronic configuration | This study |
E. coli BL21 (DE3) piBR181-At3GT. PhANS (S4) | E. coli BL21 (DE3) carrying piBR181-At3GT.PhANS in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRTrc-PhANS.At3GT (S5) | E. coli BL21 (DE3) carrying piBRTrc-PhANS.At3GT in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRTrc- At3GT. PhANS (S6) | E. coli BL21 (DE3) carrying piBRTrc-At3GT.PhANS in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRUV5-glf.glk.pgm2.galU.PhANS.At3GT (S7) | E. coli BL21 (DE3) carrying piBRUV5 vector carrying glf.glk.pgm2.galU under T7 promoter and PhANS.At3GT under lacUV5 promoter in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRUV5-glf.glk.pgm2.galU.At3GT.PhANS (S8) | E. coli BL21 (DE3) carrying piBRUV5 vector carrying glf.glk.pgm2.galU under T7 promoter and At3GT.PhANS under lacUV5 promoter in monocistronic configuration | This study |
E. coli BL21 (DE3) piBR181-glf.glk.pgm2.galU.PhANS.At3GT (S9) | E. coli BL21 (DE3) carrying piBR181-glf.glk.pgm2.galU.PhANS.At3GT in monocistronic configuration | This study |
E. coli BL21 (DE3) piBR181-glf.glk.pgm2.galU.At3GT.PhANS (S10) | E. coli BL21 (DE3) carrying piBR181-glf.glk.pgm2.galU.At3GT.PhANS in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRTrc-glf.glk.pgm2.galU.PhANS.At3GT (S11) | E. coli BL21 (DE3) carrying piBRTrc vector carrying glf.glk.pgm2.galU under T7 promoter and PhANS.At3GT under trc promoter in monocistronic configuration | This study |
E. coli BL21 (DE3) piBRTrc-glf.glk.pgm2.galU.At3GT.PhANS (S12) | E. coli BL21 (DE3) carrying piBRTrc vector carrying glf.glk.pgm2.galU under T7 promoter and At3GT.PhANS under trc promoter in monocistronic configuration | This study |