Fig. 5

The expression levels of eGFP in S. chattanoogensis L102 and L322. a The expression of eGFP in L102 (left) and L322 (right) are observed by fluorescence microscope. The observed mycelia are sampled from YEME fermentation broth at 24 h. b eGFP are detected by Western blot. Two different media are selected. TSB medium was mainly used for vegetative mycelia growth, however, YEME medium was for triggering secondary metabolite biosynthesis. pIJ8668-ermeP-egfp is used to overexpress egfp gene in Streptomyces