From: Tuning recombinant protein expression to match secretion capacity
Ā | Signal peptide | Max (IP) | IPTG (I) | Uninduced (UI) | IP/I | IP/UI | PPDA concentration (Ī¼M) at max (IP) | OD600 at max (IP) |
---|---|---|---|---|---|---|---|---|
SRP-dependent | DsbA WT | 1629 | 754 | 64 | 2 | 26 | 8 | 1āĀ±ā0 |
DsbA E1 | 2152 | 202 | 65 | 11 | 33 | 400 | 18āĀ±ā5 | |
yBG L2 WT | 4017 | 1283 | 75 | 3 | 54 | 8 | 11āĀ±ā10 | |
yBGL2 H1 | 4493 | 336 | 75 | 13 | 60 | 200 | 20āĀ±ā4 | |
SecB-dependent | Piii WT | 17 | 21 | 34 | 1 | 0 | 400 | 14āĀ±ā6 |
Piii E5 | 9818 | 607 | 77 | 16 | 127 | 400 | 24āĀ±ā3 | |
PelB WT | 1297 | 179 | 74 | 7 | 18 | 400 | 21āĀ±ā2 | |
PelB A5 | 2913 | 355 | 76 | 8 | 38 | 100 | 16āĀ±ā6 |