Tuning recombinant protein expression to match secretion capacity

Table 1 Expression performance of the BL21(LV2)-pENTRY strains containing the codon optimised signal peptide sequences

	Signal peptide	Max (IP)	IPTG (I)	Uninduced (UI)	IP/I	IP/UI	PPDA concentration (μM) at max (IP)	OD₆₀₀ at max (IP)
SRP-dependent	DsbA WT	1629	754	64	2	26	8	1 ± 0
	DsbA E1	2152	202	65	11	33	400	18 ± 5
	yBG L2 WT	4017	1283	75	3	54	8	11 ± 10
	yBGL2 H1	4493	336	75	13	60	200	20 ± 4
SecB-dependent	Piii WT	17	21	34	1	0	400	14 ± 6
	Piii E5	9818	607	77	16	127	400	24 ± 3
	PelB WT	1297	179	74	7	18	400	21 ± 2
	PelB A5	2913	355	76	8	38	100	16 ± 6

Gene expression monitored as relative fluorescence units (RFU) over optical cell density (OD) in the absence of inducer (UI), with IPTG induction only (I) and with both IPTG and PPDA induction (IP)

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