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Table 1 Expression performance of the BL21(LV2)-pENTRY strains containing the codon optimised signal peptide sequences

From: Tuning recombinant protein expression to match secretion capacity

  Signal peptide Max (IP) IPTG (I) Uninduced (UI) IP/I IP/UI PPDA concentration (μM) at max (IP) OD600 at max (IP)
SRP-dependent DsbA WT 1629 754 64 2 26 8 1 ± 0
DsbA E1 2152 202 65 11 33 400 18 ± 5
yBG L2 WT 4017 1283 75 3 54 8 11 ± 10
yBGL2 H1 4493 336 75 13 60 200 20 ± 4
SecB-dependent Piii WT 17 21 34 1 0 400 14 ± 6
Piii E5 9818 607 77 16 127 400 24 ± 3
PelB WT 1297 179 74 7 18 400 21 ± 2
PelB A5 2913 355 76 8 38 100 16 ± 6
  1. Gene expression monitored as relative fluorescence units (RFU) over optical cell density (OD) in the absence of inducer (UI), with IPTG induction only (I) and with both IPTG and PPDA induction (IP)