Fig.Ā 4From: Tuning recombinant protein expression to match secretion capacityWestern blot analysis of the periplasm (PP) and spheroplast (SP) samples from shaker flask induction. aād Precursor protein processing and spheroplast retention of scFvĪ² and scFvT were assessed with either DsbA E1 or Piii E5 signal peptide. eāh The same periplasm fractions were also assessed for the disulfide bond formation under reducing and non-reducing conditions, with (+) or without (ā) DTTBack to article page