Fig. 5
Regulated environment in 2 L bioreactor enables control over metabolic state. Fed-batch fermentation of BJ5465.sfp.bpsA with a excess glucose feed or b signal-based pulse feeding strategy resulting in glucose starvation conditions. Lines represent concentrations of total glucose fed and ethanol and acetate produced; bars represent indigoidine concentration. N = 3 technical replicates for indigoidine extraction and DCW measurements. Additional process parameters and gas analysis can be found in Additional file 1: Figures S4 and S5