Fig. 8

Profiles of N-acetyl-glucosamine (GlcNAc) and UDP-GlcNAc relative intracellular and extracellular levels when fermentations are performed at different scales and DO set points. These are key intermediates for chitin synthesis and monitored for differences over time at 10,000 L scale and DO 25% (Large_25) (orange line) and at 10 L scale at DO 5% (Small_5) (blue line), DO 8.5% (Small_8.5) (green line), and DO 12.5% (Small_12.5) (purple line). The intermediates measurement and data analysis were determined as described in “Methods” section. In this line plot graph, data are scaled such that the median value measured across all samples was set to 1.0. Error bars represents “mean +/− one standard deviation”. In the extracellular samples, GlcNAc levels are shown at “M” in the time axis for extracellular medium line plot stand for Basal Medium (light blue), and at Feed (brown) where is undetectable