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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Saccharomyces cerevisiae morphological changes and cytokinesis arrest elicited by hypoxia during scale-up for production of therapeutic recombinant proteins

Fig. 3

Quantification of cells with different DNA content by flow cytometry of fermentation at 10,000 L (10 KL) scale (dark green) and 10 L scale, within 10 L at DO 5% (orange), 8.5% (magenta), 12.5% (light green). Plots of counts of propidium iodide (PI) stained cells versus intensity of PI fluorescence at 47 (left hand-side profiles) and 82 hour (h) (right hand-side profiles) elapsed fermentation time (EFT). The DNA content is characterized by one set of chromosomes (1C), two sets (2C), up to three sets (3C) (shown by vertical arrows). For comparative purposes, all profiles at 47 h EFT were superimposed as well as those but separately at 82 h EFT. Purposely those overlays at both time points show the relative change of the subpopulations with different DNA content (1 set, 2, or 3 sets of chromosomes, or in between) as fermentations progressed to the end (82 h EFT) where the change trend is indicated by the horizontal arrow (bottom overlays)

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Microbial Cell Factories

ISSN: 1475-2859

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