Fig. 7
From: Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
In vitro enzymatic assay detected the production of crotonol catalyzed by purified enzymes. a The EIC (m/z 57) of crotonol analyzed by GC–MS. The enzymatic sample was carried out by ADHE2. b Comparison of crotonol production among three purified enzymes. The crotonyl-CoA was added as 4 mM (3.34 mg/mL). The assay time was 4 h. Data show the mean with error bars indicating standard deviation calculated from three independent biological replicates