Fig. 6
From: Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
One-pot reaction for producing crotyl diphosphate from crotonol. a Crotonol consumption, crotyl monophosphate and crotyl diphosphate production in one-pot reaction system (1.5 mg/mL of THKM82V and 0.5 mg/mL of IPK at 39.5 °C and pH 8.0). b One-pot reaction with reloading the kinases of THKM82V and IPK at 2 h and 4 h (red arrow). Data show the mean with error bars indicating standard deviation calculated from three independent biological replicates