Fig. 5
From: Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
Identification of high active THK variants and molecular docking of wild-type THK and THKM82V with crotonol. a Comparison of OD490 change between THK variants and wild-type THK. b Comparison of specific activities of purified THKM82V, THKM82V/G180R and wild-type THK. 4 mM of crotonol is added as substrate. The average value for wild-type THK is set to 1. c Molecular docking of wild-type THK and THKM82V with crotonol. Crotonol carbon atoms are colored in green and oxygen atoms in red. ATP carbon atoms are in green, oxygen atoms in red, and phosphate in orange. Loop 28–33 is in purple. The error bars represent the standard deviation of three independent repeats