Fig. 3
From: Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
In vitro enzymatic assay detecting crotyl diphosphate in M. extorquens AM1 expressing the IPK gene. a The EIC of crotyl monophosphate analyzed by LC–MS in the control assay without the addition of crude enzyme IPK (top). The EIC of crotyl monophosphate and crotyl diphosphate in the crude enzyme assay after 4 h (middle). Standards of crotyl monophosphate and crotyl diphosphate (bottom). b The increase of in vitro crotyl diphosphate and decrease of crotyl monophosphate in a time course. Data represent mean and standard deviations calculated from three biological replicates