Fig. 2
From: Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor
The production of crotyl monophosphate with crotonol feeding by M. extorquens AM1 expressing various kinase genes. a The extracted ion chromatogram (EIC) of crotyl monophosphate is analyzed by LC–MS in M. extorquens AM1 expressing erg12. Lower chromatogram is a standard of crotyl monophosphate. b crotyl monophosphate production in recombinant M. extorquens AM1. The y axis is the ratio of crotyl monophosphate between M. extorquens AM1 expressing kinase genes to the control strain carrying empty plasmid pCM80. The average value for the control strain is set to 1. Data represent mean and standard deviations calculated from three biological replicates