One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins

Table 1 Panel of resins used for affinity purification, gathering technical data provided by the manufacturers

	Manufacturer	Catalogue ref	Reference	Affinity	Nickel ligand	Features	Exclusion limit for globular proteins	Metal ion capacity/binding capacity	Max. pressure
N 1	GE Healthcare	17-5318-06	Ni Sepharose 6 Fast Flow	Nickel	NTA	6% cross-linked agarose	4000 kDa	Approx 15 µmol Ni²⁺/mL gel	0.1 MPa
N 2	ABT Agarose Beads Biotechnologies	10BCL-QHNi-5	High Density Nickel 10 BCL-QHNi-5	Nickel	IDA	10% cross-linked agarose	500 kDa	20–40 µmol Ni²⁺/mL gel	Low pressure
N 3	ABT Agarose Beads Biotechnologies	6BCL-QHNi-2	High Density Nickel 6 BCL-QHNi-2	Nickel	IDA	6% cross-linked agarose	4000 kDa	20–40 µmol Ni²⁺/mL gel	Low pressure
N 4	ABT Agarose Beads Biotechnologies	6BCL-NTANi-2	Nickel NTA agarose resin 6 BCL-NTANi-2	Nickel	NTA	6% cross-linked agarose	4000 kDa	5–19 µmol Ni²⁺/mL gel	Low pressure
N 5	ABT Agarose Beads Biotechnologies	6NiRR-2	Nickel Rapid Run 6 NiRR-2	Nickel	IDA	6% agarose	4000 kDa	5–19 µmol Ni²⁺/mL gel	0.3 MPa
N 6	ABT Agarose Beads Biotechnologies	6NTANiRR-2	Nickel NTA rapid run 6 RR-NTANi-2	Nickel	NTA	6% agarose	4000 kDa	5–19 µmol Ni²⁺/mL gel	0.3 MPa
A	NEB New England Biolabs	E8022S	Amylose resin high flow	Maltose		Cross-linked agarose	Unspecified	7 mg MBP-protein/mL gel	0.5 MPa

ISSN: 1475-2859