Fig. 8
SPR data for the assessment of MBP-16E6mut interaction properties. The protein was purified on nickel resins N1 (top) and N2 (bottom) according to the optimized protocol (5 min contact time between the bacterial extract and the affinity resin, “decant and take up” elution). a Sensorgrams for the interaction with E6APmut, plotting the response normalized by the peptide immobilization level versus time. b Sensorgrams for the interaction with peptide E6APwt, same axes. c Steady-state analysis of interaction with E6APwt, plotting the equilibrium response Req obtained in a 5-s. Window versus protein concentration