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Fig. 7 | Microbial Cell Factories

Fig. 7

From: One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins

Fig. 7

SEC chromatograms for purified MBP-E6 proteins with initial protocol, represented with dashed line (amylose resin; 2 h contact time between bacterial lysate and resin; filter elution) versus optimized protocol, represented with continuous line (N2 nickel resin; 5 min contact time; “decant and take up” elution). The elution peak corresponding to monomeric protein is indicated by the arrow. a MBP-16E6mut. b MBP-8E6

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Microbial Cell Factories

ISSN: 1475-2859

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