Fig. 5

Test of the “decant and take up” elution method on nickel resins (N1–6: Nickel; A: amylose, as detailed in Table 1). Elution was recovered by pipetting the supernatant after gentle centrifugation (5 min at 500×g) of the elution buffer–resin mixture. Monomer: black bars; oligomers: dotted bars. a MBP-16E6mut. b MBP-8E6