Fig. 4

Tests of various reticulated affinity resins (N1–6: Nickel resins; A: amylose resin, as detailed in Table 1). Elution was recovered by centrifugal filtration (5 min at 500×g) of the elution buffer–resin mixture. The ratio between monomer and total protein amount [monomer (black bars) + oligomers (dotted bar)] were calculated from the peak areas of the corresponding elution volumes. a MBP-16E6mut. b MBP-8E6