Fig. 3From: One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteinsSEC chromatograms of purified MBP-E6 proteins with the initial protocol (amylose resin; 2 h contact time between bacterial lysate and resin; filter elution). The elution peak corresponding to the monomeric protein is indicated by an arrow, according to column calibration using standard proteins. a MBP-16E6mut, b MBP-8E6Back to article page