Fig. 2

Example of analysis of purified MBP-E6 fusions by microfluidics capillary electrophoresis in denaturing conditions. a MBP-16E6mut, b MBP-8E6. The data, which normally consist in electropherograms (e.g., curves describing migrating protein signal intensity as a function of molecular size), are plotted as an intensity-based projection to mimic a conventional SDS PAGE display. Molecular weights, obtained from internal standards, are indicated in kDa. A slight shift may sometimes be observed from one lane to another, as observed in (a). These eluted samples were obtained after purification on the different resins (N1–6 for the nickel resins; A for amylose resin), 2 h contact time between bacterial lysate and resin, filter elution