Fig. 4
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12934-018-1038-0/MediaObjects/12934_2018_1038_Fig4_HTML.png)
Small scale pulsed fed-batch cultivation of E. coli W/445_Ediss in molasses medium. Sucrose concentration was monitored throughout the cultivation in regular intervals. Once the sucrose concentration was < 10 g l−1 appropriate amounts of a molasses-medium solution were pulsed to the culture to restore a sucrose concentration of 50 g l−1. Cultivation was carried out in triplicates; only one cultivation is shown for better visualization (triplicate is shown in Additional file 2: Figure S3)