A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation

Table 1 Prospecting ketoreductases from published literature

Protein name*	Protein superfamily and family	Amino acids	Organism
*FabG*	NADB Rossmann BKR like	249	*S. elongatus* [12]
Scored	NADB Rossmann SDRc	263	S. coelicolor [13]
Cmafph	NADB Rossmann MDH Like	254	C. maris [14]
LbRADH	NADB Rossmann 3β-17β HSD Like	257	L. brevis [15]
CpScrII	NADB Rossmann MDH Like	279	P. stipites [16]
Pscr	NADB Rossmann MDH Like	282	P. stipites [17]
*Hketo*	NADB Rossmann MDH Like	273	*H. polymorpha* [18]
CmagS1	NADB Rossmann MDH Like	283	C. magnoliae [19]
Kacr1	NADB Rossmann MDH Like	292	K. lactis [20]
*BYueD*	NADB Rossmann SPR like SDR	243	B. subtilis [21]
SSAkr2	NADB Rossmann AR SDR Like	323	S. salmonicolor [22]
*ZRK*	NADB Rossmann AR SDR Like	338	*Z. rouxii* [23]
LKadh	MDR FDH Like ADH3	347	L. kefir [24]
HVadh2	MDR FDH Like ADH3	349	H. volacanii [25]
HVadh1	MDR ADH6	353	H. volacanii [25]
*SsADH*	MDR Arabinose DH like	347	S. solfataricus [26]
SaADHRC3	MDR Arabinose DH like	347	S. solfataricus [27]
*PAR*	MDR Arabinose DH like	385	*Corynebacteirum* sp. [28]
SSAkr1	NADB Rossmann AR SDR Like	343	S. salmonicolor [22]

Six ketoreductases highlighted in bold were chosen from a set of 19 enzymes based on their presence in different genera and molecular weight, showing varying optomer specific catalysis based on literature
* The nomenclature used for the ketoreductases are from the references denoted in parenthesis

ISSN: 1475-2859